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Spectrophotometrical technics based assay were developed for the calculation of binding parameters like binding constant, ratio of binding constants and binding free energy of two potential antioxidants 2-(ferrocenylmethylamino)benzonitrile (2FMAB) and 3-(ferrocenylmethylamino)benzonitrile (3FMAB) with 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH). 3FMBA shows highest binding constant and highest binding free energy (2.28×10-5 M-1) and (30.06 KJmol-1) respectively. Binding free energy of DPPH-3FMAB is three times greater than that of the standard antioxidant ascorbic acid (10.93 KJmol-1)

revue des sciences fondamentales et appliquées

Determination of binding parameters of 2-(Ferrocenylmethylamino) Benzonitrile and 3-(Ferrocenylmethylamino)Benzonitrile WITH 1,1-Diphenyl-2-Picrylhydrazyl free radical