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Objectif: L’objectif de ce travail consiste a analyser les effets d’un stress hydrique et thermique sur des semis de pin pignon eleves en conditions semi-controlees. Les variations de la teneur en proline, en sucres solubles et en pigments chlorophylliens sont suivies chez les differents organes (aiguilles, tiges et racines). Methodologie et Resultats: Trois niveaux de stress hydrique sont identifies selon la saturation en eau du sol a 75%, 55% et 35% ; le stress thermique est realise a des temperatures croissantes de 38°C, 44°C, et 50°C pendant une duree de 3 heures afin de quantifier le taux des marqueurs biochimiques sur les semis âges d’une annee. Les resultats obtenus montrent une augmentation significative de la proline au niveau des aiguilles a 38°C et des taux importants de sucres solubles au niveau des aiguilles a 25°C (temoin), 38°C, 44°C et 50°C, alors qu’une diminution est remarquee pour leurs pigments chlorophylliens. Mots-cles: Pin pignon, stress, marqueurs biochimiques, proline, sucres solubles, chlorophylle English Abstract Objective: Objective of the present study was to analyze the effects of water and thermal stress on seedlings stone pine grown under semi-controlled conditions. Variations in proline, soluble sugars and chlorophyll pigments contents were monitored for different organs (leaves, stems and roots). Methodology and Results: Three levels of water stress were identified according to water saturation in the soil at 75%, 55% and 35%; the thermal stress was carried out at increasing temperatures of 38 ° C., 44 °C. and 50 ° C for a time period of 3 hours; In order to quantify the evolution of biochemical markers on oneyear-old seedlings. The obtained results showed a significant increase in rate of proline in leaves at 38 ° C; And an important rate of soluble sugars in the leaves at 25°C. (Control), 38°C, 44°C and 50 ° C, however, a decrease in their chlorophyll pigments was noticed. Keywords: Stone pine, stress, biochemical markers, proline, soluble sugars, chlorophyll

Adaptation des semis de Pin pignon (<i>Pinus pinea</i> L.) au stress combiné hydrique et thermique par des marqueurs biochimiques