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To assess the effect of stress on serum cortisol level and CD4 cell count in young male postgraduate students at Igbinedion University, a cross  sectional laboratory based analysis survey was adopted for this study. A total of 104 male volunteer postgraduate students (age 22 + 7.0 years, body mass index 26 + 0.5 kg/m2) were randomly recruited. Total white blood cell (TWBC) was determined using the Sysmex® Automated Hematology Analyzer. CD4 cell count was estimated using Partec cyflow counter. Serum cortisol level was determined by enzyme-linked  immunosorbent assay (ELISA) technique. It was observed that there was a significant (P < 0.05) increase in the serum cortisol level at stage A (1st day of the semester) when compared with stage B (midway in the semester) and C (morning of the examination), while there was significant (P < 0.05) decrease in the TWBC count and CD4 cell count at A stage when compared with stage B and C. It was observed that academic stress was inhibitory of the proliferation of CD4 cells with the elevation of serum cortisol as a possible mediator. Keywords : Stress, cortisol, CD4 cell, ELISA.

Effect of academic stress on serum cortisol level and CD4 cell count in young male postgraduate students in Okada, Nigeria