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The aim of this study was to design a glyphosate analysis method. This molecule is an organic pollutant from water and soil. We have developed a chromatographic method with phenylisothiocyanate. This molecule has allowed obtaining an intermediate molecule with the glyphosate being easily detectable in chromatography. The peak relating to this intermediate was identified using a comparison with several samples, including a blank. The tests determined the retention time (RT) of glyphosate at 1.6 min and the values of the percentage of accuracy and repeatability of the method.

Determination of glyphosate by high performance liquid chromatography (HPLC) without prior extraction