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A method for induction of multiple shoots using axillary bud and shoot tips of Sphaeranthus amaranthoides Burm. f. was described. The experiment was conducted in which multiple shoot induction was noticed on MS (Murashige and Skoog) medium supplemented with different concentrations of two cytokinins (BAP and Kin). These multiple shoots later developed into normal shoots. The highest rate of shoot proliferation was found to be 90% and 75% for axillary bud and shoot tip explants, respectively, on themedium containing BAP 4.0 mg/l. The regenerated shoots were successfully rooted on MS medium supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 90%. Keywords : In vitro, Axillary bud, Shoot tips, Multiple shoots, Regeneration, Sphaeranthus amaranthoides Burm. f. International Journal of Biological and Chemical Sciences Vol. 2 (4) 2008: pp. 579-586

Micropropagation of <i>Sphaeranthus amaranthoides</i> Burm. f. – A multipurpose medicinal herb