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Plusieurs travaux ont demontre que les vitamines A, E et K sont efficaces dans le traitement des troubles de la menstruation et de l’allaitement. L’objectif de cette etude est d’evaluer l’efficacite therapeutique des plantes galactogenes et emmenagogues utilisees dans les terroirs de la Reserve de Biosphere de la Pendjari. La Chromatographie en phase Liquide Haute Performance (CLPH) a ete utilisee pour identifier et doser les vitamines A, E et K dans ces plantes avec la methode de Billion-Rey et al. modifiee. La methode modifiee a ete adaptee et validee par sa fidelite et le taux de recuperation. Les calibrations obtenues sont lineaires avec des R² de 1 ; 0,98 et 0,99. Les teneurs en vitamine A des ecorces de Vittelaria paradoxa , de Adansonia digitata , et des graines de la variete a fleur blanche de Hibiscus sabdariffa sont respectivement 1,55 mg/g ; 0,208 mg/g et 0,08 mg/g. Les feuilles de A. digitata renferment la plus grande teneur en vitamine K (196,4 μg/g) et les graines de la variete a fleur rouge de H. sabdariffa ont la plus forte teneur en vitamine E (256 μg/g). Ces resultats montrent que ces plantes pourraient etre efficaces dans le traitement des troubles de la menstruation et de l’allaitement. © 2018 International Formulae Group. All rights reserved. Mots cles: CLPH, vitamines A, E et K, galactogenes, emmenagogues English Title: Identification and dosage of vitamins A, E and K in some plants used for the treatment of menstruation and breastfeeding disorders English Abstract Several studies have shown that vitamins A, E and K are used to solve menstrual and breast-feeding disorders. The objective of this study is to determine the therapeutic efficiency of emmenagogue and lactogenic plants most used by local populations in the Pendjari Biosphere Reserve. The High Perfomance Liquid Chromotagraphy (HPLC) was used to identify and quantify the vitamins A, E and K, in those plants with the modified method of Billion-Rey et al. The modified method was adapted and validated by his fidelity and the  recovery rate. Calibrations obtained were linear with R² equal to 1; 0.98 and 0.99. Vitamin A levels in the bark of Vitellaria paradoxa , Adansonia digitata , and seeds of the white flowered variety of Hibiscus sabdariffa are respectively 1.55 mg / g 0.208 mg / g and 0.08 mg / g. The leaves of A. digitata had the highest content of vitamin K with 196.4 mg / g. and the seeds of the red-flowered variety of H. sabdariffa had the highest value of vitamin E (256 μg / g). The results show that the studied plants could be efficient for the treatment of menstrual and breast feeding disorders. © 2018 International Formulae Group. All rights reserved. Keywords: HPLC, vitamins A, E and K, lactogenic, emmenagogue

international journal of biological and chemical sciences

Identification et dosage des vitamines A, E et K dans quelques plantes utilisées pour le traitement des troubles de la menstruation et de l’allaitement