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La recherche de matieres premieres alternatives pour la production de biopesticides microbiens suscite beaucoup d’interets. La presente etude s’est proposee d’utiliser le broyat de pericarpes des cabosses de cacao comme substrat de fermentation pour produire Bacillus thuringiensis var. kurstaki HD-1 (Btk HD-1). Les echantillons de pericarpes des cabosses ont ete collectes dans une plantation cacaoyere de Toumokro a Yamoussoukro en Cote d’Ivoire. Les parametres physico-chimiques ont ete determines selon les methodes standards. Le substrat de fermentation a ete autoclave, 2 fois successivement, a 121 °C pendant 30 min et ensemence avec 4% de l’inoculum de Btk HD-1. Il ressort de cette etude que les teneurs en carbone et en azote total ont ete respectivement de 79.7% et 1.4%. Les concentrations en mineraux du substrat ont ete plus elevees en calcium (8406.5 mg/kg) et en potassium (8248.8 mg/kg) qu’en magnesium (632.6 mg/kg), sodium (471.5 mg/kg), fer (57 mg/kg), cuivre (52.8 mg/kg), zinc (51.8 mg/kg) et manganese (44.3 mg/kg). Le denombrement des cellules et des spores viables a donne respectivement 3.2.10 11 et 3.02.10 11 UFC/ml, pour le surnageant, et 6.5.10 13 et 5.02.10 13 UFC/ml, pour le culot. Ce substrat vegetal offre une bonne perspective pour la production d’un biopesticide a base de Btk HD-1. © 2016 International Formulae Group. All rights reserved. Mots-cles: Pericarpes des cabosses de cacao, biopesticide, Bacillus thuringiensis var. kurstaki HD-1, fermentation en milieu solide, Cote d’Ivoire English Title: Use of crushed cocoa hulls as an alternative culture medium for Bacillus thuringiensis var. kurstaki HD-1 production English Abstract Research for alternative raw material for microbial biopesticides production raises outstanding interests.This study is proposed to use the crushed pericarps cocoa pods as fermentation substrate to produce  Bacillus thuringiensis var. kurstaki HD-1 (Btk HD-1). The samples pericarps pods were collected in a cocoa plantation of Toumokro in Yamoussoukro area, Cote d'Ivoire. The physico-chemical parameters were determined using standard methods. The fermentation substrate was autoclaved 2 times successively at 121 °C for 30 minutes and inoculated with 4% of Btk HD-1 inoculum. It appears from this study that the carbon and total nitrogen were 79.7% and 1.4% respectively. Mineral concentrations in substrate were higher in calcium (8406.5 mg/kg) and potassium (8248.8 mg/kg) than magnesium (632.6 mg/kg), sodium (471.5 mg/kg), iron (57 mg/kg), copper (52.8 mg/kg), zinc (51.8 mg/kg) and manganese (44.3 mg/kg). Cell counts and viable spores gave respectively 3.2.10 11 and 3.02.10 11 CFU/ml, to the supernatant, and 6.5.10 13 and 5.02.10 13 CFU/ml, for the pellet. This vegetal substrate offers a good prospect for Btk HD-1 based biopesticide production. © 2016 International Formulae Group. All rights reserved. Keywords: Cocoa hulls pod, biopesticide, Bacillus thuringiensis var. kurstaki HD-1, solid medium fermentation, Cote d’Ivoire

international journal of biological and chemical sciences

Utilisation du broyat de péricarpes des cabosses de cacao comme milieu de culture alternatif pour la production de &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; var. &lt;i&gt;kurstaki&lt;/i&gt; HD-1