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Hydrogen peroxide oxidized guaiacol to form tetramer particles that exhibited a strong resonance scattering (RS) peak at 530 nm in the presence of horseradish peroxidase (HRP) in citric acid-Na 2 HPO 4 buffer solution of pH 4.4. The RS peak increased when the concentration of hydrogen peroxide increased. The increased RS intensity (ΔI 530 nm ) was linear to the hydrogen peroxide concentration in the range of 0.55-27.6 μM, with a linear regression equation of ΔI 530 nm = 17.1 C + 1.6, a relative coefficient of 0.9996 and a detection limit of 0.03 μM H 2 O 2 . This proposed method was applied to detect hydrogen peroxide in rain water, with sensitivity, selectivity, rapidity, and recovery of 98.0-104 %. KEY WORDS : HRP, H 2 O 2 , Guaiacol, Resonance scattering spectral method Bull. Chem. Soc. Ethiop. 2011 , 25(2), 161-168.

Enzyme catalytic resonance scattering spectral detection of trace hydrogen peroxide using guaiacol as substrate