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effects of temperature (40.0 - 80.0°C), pH (6.0 - 10.0) and salt concentration (1.0, 5.0 and 10.0%) on the xylanases activity were determined. The maximum activity was obtained temperature 60.0°C and pH at 9.0. The enzyme was stable at 60.0°C for more than 60 min, suggesting that the xylanases of Bacillus strains are thermoactive and being of interests for biobleaching processes. The effectiveness of crude xylanases from the strains Ag12, Ag20 and Ag32 on kraft pulp were carried out at pH 9.0 at 60.0°C. Biobleaching studies of kraft pulp with xylanases and its subsequent treatment with 1.0% EDTA (30 min at 50.0°C) and peroxide (80 min at 70.0°C), showed that the enzymes reduced the kappa number by 27.4, 61.7 and 75.3% and enhanced the brightness by 1.0, 1.5 and 3.0% from xylanases produced by strains Ag12, Ag20 and Ag32, respectively. These results suggest that the application of this xylanases to the paper and pulp industry may be very promising.

african journal of biotechnology

Thermoactive cellulase-free xylanase production from alkaliphilic Bacillus strains using various agro-residues and their potential in biobleaching of kraft pulp