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Different mutations in the bone morphogenetic protein-15 (BMP-15) and the Growth Differentiation Factor-9 (GDF-9) genes have increased ovulation rate and infertility in a dosage-sensitive manner in sheep. To test the polymorphisms of genes in goat, which have been demonstrated as major genes of fecundity in sheep, the genetic polymorphism of FecXB and FecXG loci in BMP-15 gene were studied in 109 Iranian native goats. Blood samples were collected in EDTA coated tubes from Jugular vein and genomic DNA was extracted from whole blood samples. Single nucleotide polymorphism of FecXB and FecXG loci in BMP-15 gene were determined using PCR-RFLP technique. There was no evidence of mutation in FecXB and FecXG in these goats, all of which were monomorph for exon 2 BMP-15 gene.

Study of BMP-15 gene polymorphism in Iranian goats