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Cotyledons that affect the plant development are important part of soybean. We describe a recessive soybean mutant, designated as  curled-cotyledons  mutant which is derived from sodiumazide (NaN 3 ) and  60 Coγ ray mutagenized seeds of the ‘Nannong 94-16’ cultivar. The  curled-cotyledons  mutant has defective morphology in cotyledons development, compared to the wild-type plants. Additionally, it also has other aberrant agronomic character, such as longer growth period, and smaller plants. In the mutant, the embryo sac becomes smaller and bulbous, and ultrastructure of developing cotyledons exhibits larger vacuole, some organelles degradation, and membranous multilamellar appear at different stages. Protein and amino acid contents in seeds of mutant are higher than those of the wild type, especially methionine and cysteine. These results suggest that the  curled-cotyledons  mutant is a novel cotyledon development mutant, which could serve as a basic material to study seed composition and cotyledon development in soybean.   Keywords:  Soybean, mutant,  curled-cotyledons , genes

Characterization of a novel curled-cotyledons mutant in soybean [ Glycine max (L.) Merr.]