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Biological control using pathogenic fungi is a promising alternative to chemical control. In this study, the pathogenicity of Metarhizium anisopliae formulated with neem oil (Suneem 1%) was evaluated against Anopheles gambiae s.l. and Culex quinquefasciatus adults. Under laboratory conditions, conidia were sprayed into 30 × 30 × 30 cm netting cages at 6 × 10 7 spores/ml. With neem oil formulation, the percentages of surviving adults after 4 days were from 67±3. 4t o 5±0.5% for An. gambiae, and from 51 ± 4. 1t o 12± 1.1% for Cx quinquefasciatus. With the aqueous formulation, the survival rates were from 97 ± 3. 2t o 58± 2.1% and 95 ± 2. 5t o 70± 2.1% for An. gambiae and Cx quinquefasciatus. Very low mortality was observed in the water control. M. anisopliae in Suneem formulation could be developed for a spray technique, before being introduced in vector control.

malaria chemotherapy, control and elimination/malaria chemotherapy, control and elimination 

Evaluation of Entomopathogenic Fungus Metarhizium anisopliae Formulated with Suneem (Neem Oil) against Anopheles gambiae s.l. and Culex quinquefasciatus Adults