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Dopamine (DA) is a prominent neurotransmitter and immunomodulator with extensive biochemistry. The quantitative determination of DA can pave way for better understanding of the regulation of endocrine system, cardiovascular and renal functions. The purpose of this study was to develop a rapid, precise, and extremely sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the routine clinical quantitation of DA in urine. METHODS: Urine samples were prepared via a simple and rapid liquid-liquid extraction technique followed by a sensitive LC-MS/MS method developed using multiple reaction monitoring mode (MRM). RESULTS: The retention times of dopamine and internal standard were found to be 2.28 and 2.24 min, respectively. Mean extraction recovery of DA and DA-IS in urine was above 95.62%. DA calibration curve in urine was linear (r2 ≥ 0.998) ranging from 20 to 1000 ng/mL. The intraand inter-day precisions (RSD%) and coefficient of variation (CV%) maximum levels are 5.87 and 2.81; 10.55% and 7.57%, respectively. DISCUSSION AND CONCLUSION: A rapid, precise, sensitive, and quantitative LC-MS/MS detection of dopamine without the use of derivatisation, evaporation, and reconstitution or use of ion-pairing reagents has been developed with a non-invasive sample and easy preparation technique for clinical laboratory applications, basic neuroscience research and drug development studies.

turkish journal of pharmaceutical sciences

A Rapid, Precise, and Sensitive LC-MS/MS Method for the Quantitative Determination of Urinary Dopamine Levels &lt;i&gt;via&lt;/i&gt; a Simple Liquidliquid Extraction Technique