Open AccessUse of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis for Rapid Confirmation of Burkholderia pseudomallei in Septicemic Melioidosis
Author(s) -
Timothy J. J. Inglis,
Paul E. Healy,
Leith J. Fremlin,
Clayton L. Golledge
Publication year - 2012
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2012.11-0454
Subject(s) - burkholderia pseudomallei , melioidosis , mass spectrometry , matrix assisted laser desorption/ionization , matrix (chemical analysis) , microbiology and biotechnology , desorption , chemistry , chromatography , biology , bacteria , organic chemistry , genetics , adsorption
Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.
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