Open AccessAn Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil
Author(s) -
Luís Fábio da Silva Batista,
Marcela Segatto,
Carlos Eduardo Sampaio Guedes,
Rosana S. Sousa,
Cleusa Alves Theodoro Rodrigues,
Júlia Cristina Maksoud Brazuna,
Joselli S. Silva,
Sidney Santos,
Daniela Farias Larangeira,
Andréa Mara Macedo,
Albert Schriefer,
Patrícia Sampaio Tavares Veras
Publication year - 2012
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2012.11-0300
Subject(s) - leishmania infantum , genotyping , genetic diversity , biology , visceral leishmaniasis , genetic divergence , microsatellite , leishmania , typing , genetic variation , multilocus sequence typing , zoology , leishmaniasis , genetics , evolutionary biology , veterinary medicine , genotype , allele , population , medicine , gene , environmental health , world wide web , parasite hosting , computer science
Correlations between the genetic diversity of Leishmania infantum (syn. L. chagasi) isolates and their respective geographic origins support the theoretic assumption that visceral leishmaniasis probably originated in the Old World. Because dogs are widely considered to be the main reservoir of this disease, the present study aimed to investigate the degree of genetic divergence among 44 leishmanial canine isolates from two Brazilian cities, Jequié and Campo Grande, located approximately 2,028 km from each other. We hypothesized that a low degree of genetic divergence would be observed among these isolates. In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide support for the recent introduction of L. infantum into the New World.
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