Open AccessCorrelation between Presence of Trypanosoma cruzi DNA in Heart Tissue of Baboons and Cynomolgus Monkeys, and Lymphocytic Myocarditis
Author(s) -
James N. Mubiru,
Alice Yang,
Edward J. Dick,
Michael Owston,
R. Mark Sharp,
Jane F. VandeBerg,
Robert E. Shade,
John L. VandeBerg
Publication year - 2014
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.13-0448
Subject(s) - trypanosoma cruzi , myocarditis , chagas disease , polymerase chain reaction , biology , primer (cosmetics) , kinetoplastida , dna , pathology , virology , microbiology and biotechnology , medicine , parasite hosting , chemistry , genetics , gene , computer science , world wide web , organic chemistry
Trypanosoma cruzi, the causative agent of Chagas' disease, preferentially infects cardiac and digestive tissues. Baboons living in Texas (Papio hamadryas) and cynomolgus monkeys (Macaca fascicularis) have been reported to be infected naturally with T. cruzi. In this study, we retrospectively reviewed cases of animals that were diagnosed with lymphocytic myocarditis and used a polymerase chain reaction (PCR)-based method (S36/S35 primer set) to amplify T. cruzi DNA from archived frozen and formalin-fixed paraffin-embedded (FFPE) cardiac tissues. We show that the PCR method is applicable in archived frozen and FFPE tissues and the sensitivity is in the femtogram range. A positive correlation between PCR positivity and lymphocytic myocarditis in both baboons and cynomolgus monkeys is shown. We also show epicarditis as a common finding in animals infected with T. cruzi.
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