Open AccessSurveillance of Japanese Encephalitis Virus Infection in Mosquitoes in Vietnam from 2006 to 2008
Author(s) -
Ryusei Kuwata,
Phan Thi Nga,
Nguyễn Thị Yên,
Keita Hoshino,
Haruhiko Isawa,
Yukiko Higa,
Nguyen Huy Hoang,
Bui Minh Trang,
Loan Phuong,
Trần Vũ Phong,
Toshinori Sasaki,
Yoshio Tsuda,
Mutsuo Kobayashi,
Kyoko Sawabe,
Masahiro Takagi
Publication year - 2013
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.12-0407
Subject(s) - japanese encephalitis , virology , encephalitis , virus , biology , medicine , environmental health
Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Culex tritaeniorhynchus Giles collected in northern and southern Vietnam and two JEV strains were isolated from Culex vishnui Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their Cx. tritaeniorhynchus and Cx. vishnui.
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