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The intermediate lobe of the amphibian pituitary is poorly vascularized but receives direct aminergic and peptidergic innervations. In addition, the close contacts between the intermediate and neural lobes suggest that the latter may directly influence the activity of melanotropic cells. ln this study, we have applied the indirect immunofluorescence technique to demonstrate the presence of three novel neuro­ peptides in the neuro­ intermediate lobe complex of the frog pituitary. Fibers con­ taining atrial natriuretic factor (ANF) and melanin-concen­ trating hormone (MCH) have been visualized in the neural lobe, while numerous nerve terminais containing the neuro­ peptide tyrosine (NPY) have been observed among the parenchymal cells of the inter­ mediate lobe. These morpholo­ gical data support recent fin­ dings concerriing a role for ANF, MCH and NPY in the control of melanotropin secre­ tion in amphibians_

Localisation immunohistochimique de 3 neuropeptides (NPY ANF MCH) dans le complexe neurointermédiaire de l'hypophyse de grenouille