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We detected Legionella species in 125 samples of hot spring bath water from variousplaces in Japan using the culture and LAMP methods, and compared the results of the 2 methods. Legionella spp. was detected in 40 samples in the culture test, and 38 of these (95.0%) were also positive in the LAMP test, showing a high rate of consistency. Of the 85 negative samples in the culture test, 38 samples (44.7%) were positive in the LAMP test. The positive rate in the LAMP test was higher than that in the current culture test ; the test procedure wassimple, and judgments could be made in a few hours, showing that the LAMP method is useful for the rapid detection of Legionella spp.

biocontrol science

Comparison of Loop-Mediated Isothermal Amplification (LAMP) and Conventional Culture for the Detection of Legionella Species in Hot Spring Water Samples in Japan