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We developed 12 microsatellite loci for the endangered  minnow species, Tanichthys albonubes, using PCR-based isolation of  microsatellite arrays. These new markers were tested in 26 individuals from a  wild population collected from Guangzhou in China and 26 individuals from a  cultured strain. The number of alleles ranged from two to nine and the expected  heterozygosity from 0.177 to 0.853. The wild population had significantly  higher allelic richness than the cultured strain, with a mean allelic richness  of 5.52 (range = 3.69-8.64) and 3.13 (range = 1.99-5.73) for the wild  population and the cultured strain, respectively. No evidence of a recent  bottleneck was detected in the wild population, but it was found in the  cultured strain based on the BOTTLENECK test. These primers can be used to  understand the demography and to examine genetic differences between the  cultured T. albonubes strains and wild populations to help determine  conservation and reintroduction strategies.

genetics and molecular research

Short Communication Isolation and characterization of microsatellite markers for the White Cloud Mountain minnow (Tanichthys albonubes) in wild and cultured populations