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Although  it is a major freshwater gastropod species, genetic diversity of Bellamya  aeruginosa was completely unknown. Eighteen microsatellite loci were  isolated and characterized from (AC)(15)-enriched genomic  libraries of the freshwater snail B. aeruginosa. Most of the 18 loci  were successfully amplified and high polymorphic information content values  were found, ranging from 0.244 to 0.792 (mean 0.541). The number of alleles per  locus ranged from 5 to 13 (mean 8.8), the expected heterozygosity varied from  0.347 to 0.950 (mean 0.815) and the observed heterozygosity varied from 0.087  to 0.782 (mean 0.431). Eight loci showed significant deviation from  Hardy-Weinberg equilibrium after Bonferroni's correction and no significant  genotypic linkage disequilibrium was detected between most locus pairs, except  for TXH79-TXH97 and TXH113-TXH121. These 18 polymorphic microsatellite loci  should be useful for population genetics analysis and species identification of Bellamya.

Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)