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Wide  hybridization expands the gene pool of cultivated species and can produce  genotypes with practical value, often resistant to diseases. In plants, ISSRs  are often used for hybrid identification as well as for diversity evaluation.  We estimated genetic similarity among advanced genetic stocks of triticale  hybrids and identified Aegilops juvenalis introgressions into triticale.  Fourteen ISSR primers amplified from 12 to 23 DNA fragments. The number of  polymorphic products per primer ranged from three to nine, with a mean of 5.1.  The polymorphic information content values ranged from 0.39 to 0.66, with a  mean of 0.52. Thirteen primers amplified products indicating presence of A.  juvenalis chromatin. The largest mean genetic similarity (0.84) with all  other forms was shown by a strain derived from an A. juvenalis 6x ×  [(Lanca × L506/79) × CZR142/79] hybrid, while A. juvenalis was the least  similar (0.33). We conclude that ISSRs can reliably identify A. juvenalis chromatin  in the triticale background and efficiently estimate genetic similarity of  hybrids and parental forms.

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs