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Sex-specific DNA markers are useful for studying  sex-determination mechanisms and establishment of monosex populations. Three  widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in  China) of blunt snout bream (Megalobrama amblycephala) were screened  with AFLPs to search for sex-linked markers. Female and male pools (10  individuals in each pool) from each population were screened using 64 different  primer combinations. A total of 4789 genomic fragments were produced, with a  mean frequency of 75 bands per primer pair. Three different primer combinations  produced putative sex-associated amplifications and were selected for  individual screening in the three populations. However, none showed sex  specificity when we converted these three markers into sequence characterized  amplified region markers and evaluated all the individuals from the three  populations.

Short Communication An AFLP-based approach for the identification of sex-linked markers in blunt snout bream, Megalobrama amblycephala (Cyprinidae)