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An  interspecific artificial hybrid was produced between two economically important  aquaculture flatfish: olive flounder (Paralichthys olivaceus) and starry  flounder (P. stellatus). This hybrid displays the rapid growth  characteristic of the former and tolerance to low temperatures and low salinity  of the latter, but the genetics of inheritance in this hybrid have not been  elucidated. Polymorphic microsatellite markers developed for P. olivaceus and P. stellatus were tested to determine if these markers can be used for  analysis of parentage and genetic inheritance. Multiplex PCR using two primer  sets that were specific to each species produced PCR products of different  sizes; these could be used for the identification of interspecific hybrids.  Among the 192 primers derived from olive flounder, 25.5% of the primer sets  successfully amplified genomic DNA from starry flounder, and 23% of the 56  primer sets originating from starry flounder amplified DNA from olive flounder.  Analysis of genetic inheritance in the hybrid using seven of the 62  microsatellite markers common to both species demonstrated classic Mendelian inheritance  of these markers in the hybrid progeny, with the exception of one locus  identified as a null allele in the hybrid. These results demonstrate that  cross-specific microsatellite markers can be used tools for parentage analysis  of hybrid flatfish, for mapping quantitative trait loci, for marker-assisted  selective breeding, and for studies of the evolution of fish.

Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder