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Complex  vertebral malformation (CVM) is a recently described monogenic autosomal  recessive hereditary defect of Holstein dairy cattle that causes premature  birth, aborted fetuses and stillborn calves. Guanine is substituted by thymine  (G&gt;T) in the solute carrier family 35 member A3 gene (SLC35A3). A valine is  changed to a phenylalanine at position 180 of uridine 5'-diphosphate-N-acetyl-glucosamine  transporter protein. CVM is expected to occur in many countries due to the  widespread use of sire semen. We developed a created restriction site PCR  (CRS-PCR) method to diagnose CVM in dairy cows. This was tested on 217 cows and  125 bulls selected randomly from a Holstein cattle population in south China.  Five Holstein cows and five Holstein bulls were identified to be CVM carriers;  the percentages of CVM carriers were estimated to be 2.3, 4.0 and 2.9% in the  cows, bulls and entire Holstein cattle sample, respectively.

Identification of complex vertebral malformation carriers in Holstein cattle in south China