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An economically important marine fish species, the  giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in  Taiwan and costal areas of China. We isolated and characterized 32 polymorphic  microsatellite loci from a CA-enriched genomic library of giant grouper. The  number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed  and expected heterozygosities per locus varied from 0.387 to 1.000 and from  0.377 to 0.843, respectively. Six loci significantly deviated from  Hardy-Weinberg equilibrium. After sequential Bonferroni's correction, only two  loci showed deviation from Hardy-Weinberg equilibrium, and no linkage  disequilibrium was found between any pair of loci. These microsatellites can be  useful tools for the study of population genetics in the giant grouper.

Research Report Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae)