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Metronidazole is a derivative of benzimidazole. It is being used widely for the treatment of amoebiasis. An efficient, simple and least time consuming spectrophotometric method for the assay of metronidazole has been developed. Comparison of assay of three different brands of metronidazole has also been available in public medical stores of Karachi, Pakistan. The assay is based on the ultraviolet UV absorbance maxima at about 340 nm wavelength of metronidazole using water as solvent. A sample of drug was dissolved in water to produce a solution containing metronidazole. Similarly, a sample of ground tablets of different brands were dissolved in water and various dilutions were made. The absorbance of sample preparation was measured at 340 nm against the solvent blank and the assay was determined by comparing with the absorbance of available brand. The method can be applied for the routine QC quantitation of metronidazole in tablet formulation actively.

Simple UV Spectrophotometric Assay of Metronidazole