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A hydrophilic interaction liquid chromatography (HILIC) method coupled with a refractive index (RI) detector was developed for the determination of hydroxypropyl-β-cyclodextrin (HP-β-CD). This HILIC method used simple acetonitrile/water (80/20, v/v) as the mobile phase to provide sufficient retention for HP-β-CD. The method was found to be specific without interference from the other constituents in pharmaceutics. The method was also validated for linearity and accuracy of the drug sample. The effects of various parameters, such as acetonitrile content, mobile phase pH, and column temperature on HILIC analysis were investigated. Furthermore, the retention mechanism was assessed by the evaluation of the common chromatographic parameters.

A HILIC Mechanism Discussion for the Retention of HP-<i>β</i>-CD