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To utilize starch and protein contained in microalgae as carbon and nitrogen sources for ethanol production, an extraction method, i.e. ultrasonic treatment using a homogenizer, and simultaneous saccharification and fermentation (SSF) of extracted microalgae solution were studied using <i>Chlamydomonas fasciata</i> Ettl 437. 30 min of ultrasonic treatment gave the maximum extraction ratio of starch contained in microalgae, i.e. 93.8%, that corresponded to 0.408 g-starch/g-dry microalgae. SSF of the extracted solution obtained from ultrasonic treated microalgae at 30 min by glutase-AN and <i>Saccahromyces cerevisiae</i> AM12 provided 0.194 and 0.168 g-ethanol/g-dry microalgae with and without yeast extract, respectively, corresponding to 79.5 and 68.8% of theoretical ethanol yield

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Efficient Extraction of Starch from Microalgae Using Ultrasonic Homogenizer and Its Conversion into Ethanol by Simultaneous Saccharification and Fermentation