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Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of us- ing this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, in- cluding the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic condi- tions using either a batch or a fed-batch process at a total water-insoluble solids loading of 10%. Overall yields of etha- nol based on all available sugars of 0.24 g/g and 0.27 g/g were obtained for batch and fed-batch mode of operation, re- spectively. The higher yield in the fed-batch process coincided with a higher degree of conversion of the sugars in the liquid medium, in particular of arabinose, for which the conversion was doubled (from 48% to 97%)

journal of sustainable bioenergy systems

Fermentation of the Straw Material Paja Brava by the Yeast &amp;lt;i&amp;gt;Pichia stipitis&amp;lt;/i&amp;gt; in a Simultaneous Saccharification and Fermentation Process