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The second dissociation constant, pK2, and related thermodynamic quantities for TAPSO have been previously determined and reported from the temperatures (278.15 to 328.15) K. In the current study there are five buffer solutions without NaCl and five buffer solutions with NaCl present which yield an ionic strength (I = 0.16 mol·kg-1) similar to that of blood plasma. These buffer solutions have been evaluated in the temperature range of (278.15 to 328.15) K using the extended Debye-Hückel equation, due to the limitations of the Bates-Guggenheim convention such that it is only valid when I < 0.1 mol·kg-1. The liquid junction potential (Ej) values between the TAPSO solution and the saturated KCl calomel electrode solution have been estimated at (298.15 and 310.15) K using a flowing junction cell measurement. The previously mentioned Ej values have been used in determining the operational pH values at (298.15 and 310.15) K. These TAPSO buffer solutions are recommended as reference solutions for pH measurements in saline media with an ionic strength of I = 0.16 mol·kg-1

journal of biophysical chemistry

Buffer standards for the physiological pH of the zwitterionic buffer 3-[&amp;lt;i&amp;gt;N&amp;lt;/i&amp;gt;-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic Acid (TAPSO) From (278.15 to 328.15) K