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Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.

Heat-Resistant Properties of &lt;i&gt;&amp;#945&lt;/i&gt;-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

Heat-Resistant Properties of <i>&#945</i>-Chymotrypsin Adsorbed onto Biomass Charcoal Powder