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The sambong (Blumea balsamifera (L.) DC.) leaf was investigated by applying frozen section and thin section technology, and observed through optical microscope. The results showed that the sambong leaf was firstly originated nearby the apical bud at the stem tip. The first leaf grows fast, that the mitosis happens frequently and the cytoplasm manufacture rapidly. The cells in the first few leaves have not yet differentiated into the spongy parenchyma and palisade parenchyma. In the mature leaf, the spongy parenchyma and palisade parenchyma were fully developed. The epidermal hairs were firstly developed on the abaxial side of the first leaf.

Morphological Development of Sambong (Blumea balsamifera (L.) DC.) Leaf Studied by Frozen Section and Thin Section