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Semi-large scale liquid cultivation of transformed Escherichia coli (E. coli) in medium (100-200 ml) has been widely used for the acquisition of relatively large amounts of plasmid DNA (50-300 μg). However, this method requires an expensive high-speed centrifugation apparatus to precipitate E. coli before lysis, which is both laborious and time-consuming. Here, we demonstrate a method for agar plate-based cultivation of bacteria that does not employ a high-speed centrifugation apparatus. This procedure proves to be simple and reproducible, yielding an average of 82 μg of plasmid DNA per experiment. It may therefore be valuable for cloning/transfection experiments under limited financial backgrounds

A simplified protocol for the semi-large scale recovery of plasmids from &lt;i&gt;Escherichia coli&lt;/i&gt; grown on agar plates

A simplified protocol for the semi-large scale recovery of plasmids from <i>Escherichia coli</i> grown on agar plates