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Microfluidic behavior of ternary mixed carrier solvents of water-acetonitrile-ethyl acetate (2:3:1 volume ratio) was examined by use of a microchip incorporating microchannels in which one wide channel was separated into three narrow channels, i.e., triple-branched microchannels. When the ternary carrier solution containing the fluorescent dyes, hydrophobic perylene (blue) and relatively hydrophilic Eosin Y (green), was fed into the wide channel under laminar flow conditions, the carrier solvent molecules or fluorescence dyes were radially distributed in the channel, forming inner (organic solvent-rich major; blue) and outer (water-rich minor; green) phases in the wide channel. And then, in the narrow channels, perylene molecules mostly appeared to flow through the center narrow channel and Eosin Y, which is distributed in the outer phases in the wide channel, flowed through the both side narrow channels. A metal ion, Cu(II) as a model, dissolved in the ternary mixed carrier solution was also examined. The Cu(II) showed fluidic behavior, transferring from the homogeneous carrier solution to the water-rich solution in the side narrow channels through the triple-branched microchannels

Microfluidic Behavior of Ternary Mixed Carrier Solvents Based on the Tube Radial Distribution in Triple-Branched Microchannels in a Microchip