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In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.

american journal of plant sciences

Expression Vector Construction and Genetic Transformation of &amp;lt;i&amp;gt;Rosa rugosa β&amp;lt;/i&amp;gt;-l,3-Glucanase Gene (&amp;lt;i&amp;gt;RrGlu&amp;lt;/i&amp;gt;)