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Genome wide analysis of Arabidopsis thaliana reveals a unique genetic arrangement of ACGT motif in the protein phosphatase <st1:chmetcnv unitname="C" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">2C</st1:chmetcnv> (PP<st1:chmetcnv unitname="C" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">2C</st1:chmetcnv>) like promoter (accession number AT<st1:chmetcnv unitname="g" sourcevalue="5" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">5G</st1:chmetcnv>59220). In the present study, full length, 900 base pair, 500 base pair, 400 base pair and NRM deletion variants of PP<st1:chmetcnv unitname="C" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">2C</st1:chmetcnv> like promoter were constructed to investigate the activity of PP<st1:chmetcnv unitname="C" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">2C</st1:chmetcnv> like promoter in the presence of inducers like abscisic acid, jasmonic acid and salicylic acid. The PP<st1:chmetcnv unitname="C" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0" w:st="on">2C</st1:chmetcnv> promoter has three ACGT elements in close vicinity. They are so positioned that they are separated by a spacer of 30 base pair and 5 base pair respectively from each other. The study has shown that ACGTN30ACGT genetic architecture is essential for the promoter to be induced in response to abscisic acid. The synergistic and antagonistic effects of cis elements were observed. AACA is a positive regulatory element in endosperm and is known to act as negative regulatory element in other tissues. In this study AACA, have been found to negatively regulate the expression of reporter gene EGFP in both induced and under uninduced conditions.<o:p></o:p> </p

american journal of plant sciences

AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (&amp;lt;i&amp;gt;PP2C&amp;lt;/i&amp;gt;) like Promoter in &amp;lt;i&amp;gt;Arabidopsis thaliana&amp;lt;/i&amp;gt;