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A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).  </p

american journal of molecular biology

Breeding of NHase hyper-producing &amp;lt;i&amp;gt;Rhodococcus ruber&amp;lt;/i&amp;gt; strain LUV&amp;lt;sub&amp;gt;30-06&amp;lt;/sub&amp;gt; and verification of mutants by RAPD