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Many microorganisms can tolerate high-temperature ranges from 37°C - 45°C are called thermotolerant microorganisms. Eighteen such isolates containing various microorganisms were collected from the natural fermented products of Bangladesh in summer for bioethanol production. Cultural, morphological, physiological, biochemical and genetical analysis were carried out under various physiological conditions. Among them, two thermotolerant strains Tari-6, isolated from the Tari (an overnight natural fermented palm juice at around 33°C - 40°C) and Pvt-1, isolated from the Pantavat (an overnight natural fermented rice soaked with tap water at around 35°C - 37°C), produced high amount of bioethanol, 7.5% (v/v) and 6.5% (v/v), respectively at 37°C. Furthermore, a partial 26S rDNA sequencing results confirmed that the Tari-6 and Pvt-1 encoded Pichia galeiformi and Pichia guilliermondii, respectively, and later one could grow well in media containing Xylose. Our results conclude that these two yeast strains are the potential candidates for bioethanol production.

Production of Bioethanol at High Temperature from Tari