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Objective: Evaluating the bio-reducing activity and variouspotential, annotates the biological entitties of Fagonia cretica. Method: By fractionating the crude extractwith different ethanol-water fraction mixture. This substantiates the reductionpotential for the biological synthesis of AgNPs, its antioxidant, anti-ureaseand anti-Tyrosinase activity. Results: UV-visible spectra confirm thebio-reducing potential for Ag NPs with the optimized 50% ethanol-water fractionmixture. Transmission electron microscopy (TEM) confirmed the round shapedAgNPs with an average size of 16 nm in optimized 50% fraction mixture. Furtherevaluating Ethanol-water fraction with 90% and 70% of plant extract showed morepotentials for Anti-Tyrosinase and Anti-urease activity. Moreover, diluted 30%fraction exhibits two folds higher Anti-oxidant and Anti urease activity. Conclusion: Overall, this work showed that 50% Ethanol-water fraction has potentiallyactive molecules (among all fractions) primarily involved in bio-reducingpotential of Ag NPs and enzymatic assay while 90% mixture proved to be leastactive. This study suggested electing the optimum mixture fraction of 50%ethanol-water in order to screen the bioactive compounds and to inculcate theiractivity status for clinical trials.

advances in bioscience and biotechnology

Intrinsic Bio-Enhancer Entities of &amp;lt;i&amp;gt;Fagonia cretica&amp;lt;/i&amp;gt; for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity