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Bacterial contamination of air may have human health implications by the transmission of potential human pathogens. Therefore, assessment of air bacterial abundance and composition in different built environment is essential. Jawaharlal Nehru University health centre (UHC) is a primary healthcare setting providing need-based medication to university students. Using active air sampling method, we collected eight air samples from the indoor and outdoor area of UHC across four different seasons. The total genomic DNA was extracted from the air samples and subjected to 16S rRNA gene-based next-generation sequencing. We performed the taxonomic classification along with comparative analysis of air bacterial communities. This study revealed that Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes are the dominant phyla in the sampled air. Overall, the air bacterial composition in our studied samples was comparatively simple; only ten taxonomic families accounting for ~75% of the total sequences determined. We also observed ESKAPE pathogens in the air metagenomes in a low percentage (4.42%), which were dominated by Pseudomonas, Acinetobacter and Staphylococcus. Proteobacteria, Actinobacteria and Firmicutes showed significant correlation with PM2.5. We suggest that routine air monitoring and microbiological survey is essential for air quality standards and potential human pathogens detection in health care settings. It is the first report from India to uncover the temporal dynamics of air bacterial communities in UHC using Illumina MiSeq (PE300) sequencing and Quantitative Insights into Microbial Ecology (QIIME).

Temporal Dynamics of Air Bacterial Communities in a University Health Centre Using Illumina MiSeq Sequencing