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Serum progesterone level is used as an indictor of ovulation and several assays are commercially available for its routine measurement. Target values suggestive of ovulation, deficient luteal phase and anovulation have been proposed and are widely sighted in the literature. In this study, we com- pared the use of two different assays namely: Amerlite (Johnson & Johnson, United Kingdom), and AxSym (Abbott Diagnostics, Germany) for measure- ment of serum progesterone. In patients being investigated for infertility (n = 93), serum progesterone levels showed good correlation (r = 0.92) between the two methods; however, values were significantly different (P < 0.005). Using published cut-offs levels for progesterone, 28.3%, 60.8%, and 10.9% of pa- tients had ovulatory, anovulatory, ad deficient luteal phase, respectively, as measured by the Amerlite assay, compared to 32.6%, 60.8%, and 6.5% when using the AxSym assay. Thus when using the AxSym assay, higher per- centage of patients would be classified as having ovulaory cycle. This study indicated that methodological differences on their own right play a significant role in the determination of ovulatory statue of patients. The study showed that laboratory-based target values need to be established.

Circulating Levels of Progesterone as Biochemical Evidence of Ovulation is Method Dependent