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Fast, sensitive, precise and selective high performance liquid chromatography and high performance thin layer chromatography methods were developed for the simultaneous estimation of curcumin and quercetin in a polyherbal formulation. Validation of those methods was carried out with respect to linearity, range, precision, accuracy and robustness. Both methods were applied to a polyherbal formulation to find the amount of curcumin and quercetin and compare. Curcumin and quercetin were found to be 9.5 mg and 0.613 mg in the polyherbal formulation using the high-performance liquid chromatography method whereas with the high performance thin layer chromatography method, curcumin and quercetin were found to be 9.7 mg and 0.674 mg, respectively. Both methods identified and quantified curcumin and quercetin effectively.

HPLC and HPTLC methods for Simultaneous Estimation of Quercetin and Curcumin in Polyherbal Formulation