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The Natural Herbal Oil Formulation (NHOF) have been designed to work against White Spot Syndrome Virus (WSSV) in Shrimp. The essential oil blend extracted from the following plants, Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus, mixed together to develop as anti-WSSV product. The product were added in the feed, as feed additive. Another product developed which was applied in tank water as water supplement named as “Pondguard” (Reg no. KKP RI no. D16060285-HBC). The Pondguard is composed of three essential oils i.e., Eucalyptus globulus, Pinus sylvestris and Lavandula latifolia. A bioassay trial was conducted to determine the efficacy of developed formulation as an anti-WSSV candidate. The experimental shrimp was fed on NHOF-mixed feed and “Pondguard” applied in the tank water whereas control shrimp were fed on the regular feed. The shrimp of both experimental and control were challenged by WSSV on 15th day of feeding. Two methods of WSSV challenge were applied, per-os challenge and immersion challenge. The cumulative mortality in positive control reached up to 100% on DPI 10 whereas no mortality appeared in the experimental group and in negative control. The trial results show that the developed natural herbal formulation has significant effect against WSSV in a controlled condition.

Effectiveness of Natural Herbal Oil Formulation against White Spot Syndrome Virus in Penaeus vannamei