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Clinical islet transplantation is a well-established and a relatively safe procedure for diabetic patients, whereas autologous islet transplantation is performed as an adjunctive therapy following a total or partial pancreatectomy from patients with chronic pancreatitis. Successful clinical human allo or auto-islet transplantation requires recovery of a suU¹cient number of functional islets from cadaveric or chronic pancreatitis pancreata. During the last two decades, significDnt progress has been made in the islet isolation procedures and in the use of tissue dissociation enzymes. However, it is still challenging to recover all available islets from pancreas. In many centers, about 50% of the islet isolations do not generate a suU¹cient number of islets for single donor transplants. Poor islet recovery remains, a critical issue which must be addressed as to achieve widespread adoption of islet transplantation.

Role of Tissue Dissociation Enzymes on Human Islet Yield Intended for Clinical Islet Cell Transplantation