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A pectinase-ultrasound synergistic extraction (PUSE) method was developed to extract chlorogenic acid (CGA) from Flos Lonicerae Japonicae (FLJ). The screening and optimization experiments revealed that 20% (v/v) ethanol concentration in conjunction with an ultrasonic power 90 W was the optimal condition. Moreover, other parameters including pectinase concentrations, liquid-solid ratio, ultrasonic time and pH were systematically optimized. The scanning electronic micrographs revealed that the treatments with pectinase and ultrasound distinctly facilitated the extraction process, in which the plant cell wall was destructed in large extent. Compared with other previously reported methods, PUSE was more efficient with higher CGA extraction yield and CGA yield can reach 5.13%.

Pectinase-Ultrasound Synergistic Extraction of Chlorogenic Acid from Flos Lonicera Japonicae