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Cell lines have been established from different fish species especially for virus isolation and for studying cell-pathogeninteractions, and therefore are of interest in aquaculture. In this paper, we have investigated the presence and theregulation of some immune genes in the DLEC (Dicentrarchus labrax embryonic cells) cell line from European sea bass(Dicentrarchus labrax L.) to preliminary elucidate their action. The basal expression of the selected genes (interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), transforming growth factor-β (TGF-β), CD8-α, major histocompatibility complexII-β (MHC II-β), interferon (IFN) and Mx protein (Mx)) have been investigated and, successively, their modulation havebeen studied both after stimulation with different mitogen agents and after a transfection with a sequence codifying forthe coat protein of a fish nervous necrosis virus (NNV). The results have evidenced that the inflammatory molecules(IL-1β, COX-2, TGF-β), constitutively expressed by the DLEC cell line, are not up-regulated by the stimulation withlipopolysaccharide (LPS) from E. coli, whether the expression of the T-cell marker transcripts (CD8-α, MHC II-β) isinfluenced by the action of a lectin from Phaseolus vulgaris (PHA-L). Finally, the expression of the coat NNV proteinin the DLEC cell line, after the transfection, led to an high up-regulation of IFN and Mx gene transcripts. These datasuggest that the DLEC cell line recognize specific pathogen-associated molecular patterns (PAMPs) and, therefore,could be useful for studying T-cell pathways and viral responses in sea bass avoiding the use of live test animals

journal of aquaculture research and development

Analysis of the Expression and Modulation of Selected mmunerelated Gene Transcripts in the DLEC Cell Line from European Sea Bass (Dicentrarchus labrax L.)