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Development of new technologies for Biological Warfare Agents (BWA) stand-off detection implies several\udsafeties, logistic and economic drawbacks that involve production of different highly virulent bacteria and viruses,\udtheir isolation and characterization under adequate bio-containment and sample preparation for each agent to\udevaluate the testing method. In order to overcome these difficulties most of the research activities and tests reported\udso far, are performed using simulants: Biological Agents (BA) which are phylogenetically or structurally related to\udBWA. The use of the simulants (BWA-S) show, however, some limitations: they can share some of the properties of\udthe biological warfare agents but have different antigens, proteome and genome. In this work, different BWA-S was\udevaluated for the application in the development and training of stand-off detection systems. This study is the basis\udfor the use of simulants in the development of an Ultraviolet Laser Induced Fluorescence (UV-LIF) based detection systems

Use of Non-Pathogenic Biological Agents as Biological Warfare Simulants for the Development of a Stand-Off Detection System