English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The peptidyl-prolyl isomerase Pin1 is overexpressed in many human cancers, including melanoma. To investigate its possible role in oncogenesis of melanoma and as a therapeutic target, we suppressed Pin1 expression in the human melanoma cell line A375 by microRNA (miRNA) interference technology. Two stable clones with suppressed Pin1 were established by stable transfection of miRNA plasmid targeting Pin1 into A375 cells. Both clones showed reduced proliferation and invasion in vitro and suppressed tumorigenic potential in athymic mice. Furthermore, Pin1 inhibition also resulted in decreased phosphorylation of Akt and repressed expression of C-Jun N-terminal kinase and pro-matrix metalloproteinase 2, which were associated closely with the development of melanoma. These findings indicate that Pin1 plays an important role in the tumorigenesis of melanoma and might serve as a promising therapeutic target.

RNA-interference-mediated downregulation of Pin1 suppresses tumorigenicity of malignant melanoma A375 cells