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Picobirnaviruses (PBVs) were initially discovered in the fecal specimens in humans during investigation of children gastroenteritis causative agent in Brazil in 1988 (1). PBVs are found in the feces from rats, rabbits, simian and others, thus, PBVs appear to infect a very extensive range of hosts (2, 3, 4). PBVs belong to the family Picobirnaviridae, which are a group of small, non-enveloped viruses measuring 35–40 nm in diameter with bi-segmented dsRNA genome. The larger genomic segment, or segment 1 ranges from 2.2 to 2.7 kbp, encoding for the capsid protein, and the smaller one, or segment 2 ranging from 1.2 to 1.9 kbp, encoding for the RNAdependent RNA polymerase (RdRp) (5). PBVs are classified into genogroup I (prototype 1-CHN-97) and II (prototype 4-GA-91) according to the segment 2 genome (6, 7, 8). Here, we amplified nearly complete segment 2 nucleotide sequences of a genogroup II picobirnavirus strain from swine fecal specimen using 4 sets of primers (Tab.1) designed according to picobirnavirus 4-GA-91 strain (prototype) available in GenBank (AF246940). Fecal specimens were resuspended in PBS (0.01 M phosphate, pH 7.2–7.4) to make 20% (w/v) solution for RNA extraction and centrifuged at 12,000 × g for 5 min at room temperature. Total RNA was extracted from 300 μl supernatant by using TRIZOL reagent (Invitrogen), according to LETTER TO THE EDITOR

Segment 2 sequences analysis of genogroup II picobirnavirus in pig stool in China